What is the integrity of your RNA?

April 19, 2011 18:06 Posted by David Vaudry

Isolation of intact RNA is a critical first step in obtaining meaningful gene expression data. Working with low-quality RNA may strongly compromise your qRT-PCR results. To verify RNA quality automated capillary-electrophoresis systems are now available. Profiles generated provide key information on RNA concentration and RNA integrity. The Agilent 2100 Bioanalyzer (Agilent Technologies, USA) provides a framework for the control of RNA quality. RNA quality can be quantified with the RNA Integrity Number (RIN) provided by the Bioanalyzer. Samples are evaluated using 6 parameters and a value ranging from 1 to 10 is assigned. The higher the RIN, the better the integrity of your sample. For more information on the impact of RNA degradation on gene expression profiles, click here.

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