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What is the integrity of your RNA?

April 19, 2011 18:06 Posted by David Vaudry

Isolation of intact RNA is a critical first step in obtaining meaningful gene expression data. Working with low-quality RNA may strongly compromise your qRT-PCR results. To verify RNA quality automated capillary-electrophoresis systems are now available. Profiles generated provide key information on RNA concentration and RNA integrity. The Agilent 2100 Bioanalyzer (Agilent Technologies, USA) provides a framework for the control of RNA quality. RNA quality can be quantified with the RNA Integrity Number (RIN) provided by the Bioanalyzer. Samples are evaluated using 6 parameters and a value ranging from 1 to 10 is assigned. The higher the RIN, the better the integrity of your sample. For more information on the impact of RNA degradation on gene expression profiles, click here.

Click here to see The Good, The Bad and The Ugly of RNA quality

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Training session on high throughput qPCR

April 19, 2011 15:14 Posted by David Vaudry

PRIMACEN will organize from November 23 to 25, a training session on high throughput qPCR (96 to 1536 samples) to analyze gene expression. The program includes primer design, RNA extraction, reverse transcription, PCR and data analysis. Five hours for the theory and 18 hours of practice. For more information, please contact us.

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